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Issue: Vol. 5361 No. 1: 31 Oct. 2023
Type: Article
Published: 2023-10-31
DOI: 10.11646/zootaxa.5361.1.2
Page range: 53-73
Abstract views: 348
PDF downloaded: 23

Description of two new species in the Ixodes ricinus complex from the New World (Acari: Ixodidae), and redescription of Ixodes affinis Neumann, 1899

Instituto de Investigaciòn de la Càdena Làctea (IDICAL; INTA-CONICET); Instituto Nacional de Tecnología Agropecuaria; Estaciòn Experimental Agropecuaria Rafaela (INTA E.E.A. Rafaela); Ruta 34 Km 227; Rafaela; Santa Fe; Argentina
United States National Tick Collection; Institute for Coastal Plain; Science; Georgia Southern University; Statesboro; GA 30460; USA
Laboratorio de Vectores y Enfermedades Transmitidas; Departamento de Ciencias Biológicas; CENUR Litoral Norte; Universidad de la República; Rivera 1350; 50000 Salto; CP; Uruguay
Department of Biology; Georgia Southern University; Statesboro; GA 30460; USA
Instituto Conmemorativo Gorgas de Estudios de la Salud; Panama
Instituto de Investigaciòn de la Càdena Làctea (IDICAL; INTA-CONICET); Instituto Nacional de Tecnología Agropecuaria; Estaciòn Experimental Agropecuaria Rafaela (INTA E.E.A. Rafaela); Ruta 34 Km 227; Rafaela; Santa Fe; Argentina
Instituto de Investigaciòn de la Càdena Làctea (IDICAL; INTA-CONICET); Instituto Nacional de Tecnología Agropecuaria; Estaciòn Experimental Agropecuaria Rafaela (INTA E.E.A. Rafaela); Ruta 34 Km 227; Rafaela; Santa Fe; Argentina
Georgia Southern Museum; Georgia Southern University; Statesboro; GA 30460; USA
Zoologische Staatssammlung München; München; Germany
Instituto de Investigaciòn de la Càdena Làctea (IDICAL; INTA-CONICET); Instituto Nacional de Tecnología Agropecuaria; Estaciòn Experimental Agropecuaria Rafaela (INTA E.E.A. Rafaela); Ruta 34 Km 227; Rafaela; Santa Fe; Argentina
Acari Ixodes taxonomy New World new species

Abstract

Ixodes chacoensis n. sp. is described based on males, females, nymphs and larvae collected from vegetation, ungulates and passerine birds in northeastern Argentina. Ixodes affinis Neumann, 1899 is redescribed based on the original type specimens (females) from Leopardus pardalis, and from recently collected specimens from Costa Rica. Ixodes keiransi n. sp., previously treated as North American populations of Ixodes affinis, is described based on males and females from carnivores and ungulates from the southeastern United States. Concatenated total evidence phylogenetics based on combined DNA sequence analyses from mitochondrial genes (12SrDNA, 16SrDNA and COI) and a nuclear gene (ITS2) corroborate the recognition of these species.

 

References

  1. Backus, L.H., Foley, J.E., Hobbs, G.B., Bai, Y. & Beati, L. (2022) A new species of tick, Ixodes (Ixodes) mojavensis (Acari: Ixodidae), from the Amargosa Valley of California. Ticks and Tick-Borne Diseases,13, 102020. https://doi.org/10.1016/j.ttbdis.2022.102020
  2. Beati, L. & Keirans, J.E. (2001) Analysis of the systematic relationships among ticks of the genera Rhipicephalus and Boophilus (Acari: Ixodidae) based on mitochondrial 12S ribosomal DNA gene sequences and morphological characters. Journal of Parasitology, 87, 32–48. https://doi.org/10.1645/0022-3395(2001)087[0032:AOTSRA]2.0.CO;2
  3. Beati, L., Patel, J., Lucas-Williams, H., Adakal, H., Kanduma, E.G., Tembo-Mwase, E., Krecek, R., Mertins, J.W., Alfred, J.T., Kelly, S. & Kelly, P. (2012) Phylogeography and demographic history of Amblyomma variegatum (Fabricius) (Acari: Ixodidae), the tropical bont tick. Vector-Borne and Zoonotic Diseases, 12, 514–525. https://doi.org/10.1089/vbz.2011.0859
  4. Bernegossi, A.M., de Souza Borges, C.H., Sandoval, E.D.P., Cartes, J.L., Cernohorska, H., Kubickova, S., Vozdova, M., Caparroz, R., González, S. & Barbanti Duarte, J.M. (2022) Resurrection of the genus Subulo Smith, 1827 for the gray brocket deer, with designation of a neotype. Journal of Mammalogy, gyac068. https://doi.org/10.1093/jmammal/gyac068
  5. Bermúdez, S., Apanaskevich, D.A. & Domínguez, L. (2018) Garrapatas Ixodidae de Panamá. Secretaría Nacional de Ciencia,Tecnología e Innovación, Panamá, 129 pp.
  6. Clifford, C.M. & Anastos, G. (1960) The use of chaetotaxy in the identification of larval ticks (Acarina: Ixodidae). Journal of Parasitology, 46, 567–578. https://doi.org/10.2307/3274939
  7. Cooley, R.A. & Kohls, G.M. (1945) The genus Ixodes in North America. National Institute of Health Bulletin, 184, 1–246.
  8. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9, 772. https://doi.org/10.1038/nmeth.2109
  9. Folmer, O., Black, M., Hoeh, W., Lutz, R., Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
  10. Gerrish, R.R. & Ossorio, J.M. (1965) New record of the tick Ixodes affinis Neumann in the United States. Journal of Economic Entomology, 58, 369. https://doi.org/10.1093/jee/58.2.369
  11. Guglielmone, A.A. & Nava, S. (2014) Names for Ixodidae (Acari: Ixodoidea): valid, synonyms, incertae sedis, nomina dubia, nomina nuda, lapsus, incorrect and suppressed names—with notes on confusions and misidentifications. Zootaxa, 3767 (1), 1–256. https://doi.org/10.11646/zootaxa.3767.1.1
  12. Guglielmone, A.A., Sánchez, M.E., Franco, L.G., Nava, S., Rueda, L.M. & Robbins, R.G. (2015) continuously updated) Hard ticks (Acari: Ixodida: Ixodidae): a non-profit open-access web portal for original descriptions of tick species (valid and invalid), dubious and uncertain names, and selected nomina nuda. Available from: http://rafaela.inta.gob.ar/nombresgarrapatas (accessed 8 August 2023)
  13. Guglielmone, A.A., Nava, S. & Robbins, R.G. (2021) Neotropical hard ticks (Acari: Ixodida: Ixodidae). A critical analysis of their taxonomy, distribution, and host relationships. Springer Nature Switzerland AG, Cham, 486 pp. https://doi.org/10.1007/978-3-030-72353-8
  14. Guglielmone, A.A., Nava, S. & Robbins, R.G. (2023) Geographic distribution of the hard ticks (Acari: Ixodida: Ixodidae) of the world by countries and territories. Zootaxa, 5251, 274 pp.
  15. Hall, T.A. (1999) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
  16. Herbert, P.D.N., Cywinska, A., Ball, S.L. & de Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceeding of the Royal Society of London B, 270, 313–321. https://doi.org/10.1098/rspb.2002.2218
  17. Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755. https://doi.org/10.1093/bioinformatics/17.8.754
  18. Keirans, J.E. & Clifford, C.M. (1978) The genus Ixodes in the United States: a scanning electron microscope study and key to adults. Journal of Medical Entomology, Supplement 2, 1–149. https://doi.org/10.1093/bioinformatics/17.8.754
  19. Keirans, J.E., Needham, G.R. & Oliver, Jr J.H. (1999) The Ixodes ricinus complex worldwide: diagnosis of the species in the complex, hosts and distribution. In: Needham, G.R., Mitchell, R., Horn, D.J. & Welbourn, W.C. (Eds.), Acarology IX. Vol. 2. Symposia. Ohio Biological survey, Columbus, Ohio, pp. i–xvii + 1–507.
  20. Kohls, G.M. & Rogers, A.J. (1953) Note on occurrence of the tick Ixodes affinis Neumann in the United States. Journal of Parasitology, 39, 669. https://doi.org/10.2307/3274091
  21. Labruna, M.B., Onofrio, V.C., Barros-Battesti, D.M., Gianizella, S.L., Venzal, J.M. & Guglielmone, A.A. (2020) Synonym of Ixodes aragaoi with Ixodes fuscipes, and reinstatement of Ixodes spinosus (Acari: Ixodidae). Ticks and Tick-borne Diseases, 11,101349. https://doi.org/10.1016/j.ttbdis.2019.101349
  22. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X. Version 2.0. Bioinformatics, 23, 2947–2948. https://doi.org/10.1093/bioinformatics/btm404
  23. Lund, H.O., Marshall, C.M. & Hayes, F.A. (1962) The occurrence of Ixodes affinis Neumann on Blackberad Island, Georgia (Acarina: Ixodidae). Proceeding of the Entomological Society of Washington, 64, 105–106.
  24. Maddison, W.P. & Maddison, D.R. (2018) Mesquite: A Modular System for Evolutionary Analysis. Version 3.51. Available from: http://www.mesquiteproject.org (accessed 8 August 2023)
  25. Mangold, A.J., Bargues, M.D. & Mas-Coma, S. (1998) Mitochondrial 16S rRNA sequences and phylogenetic relationships of Rhipicephalus and other tick genera among Metastriata (Acari: Ixodidae). Parasitology Research, 84, 478–484. https://doi.org/10.1007/s004360050433
  26. Mclain, D.K., Wesson, D.M., Collins, F.H. & Oliver, Jr. J.H. (1995) Evolution of the rDNA spacer, ITS 2, in the ticks Ixodes scapularis and I. pacificus (Acari: Ixodidae). Heredity, 75, 303–319. https://doi.org/10.1038/hdy.1995.139
  27. Nadolny, R.M., Gauthier, D.T., Gaff, H.D. & Bermúdez, S.E. (2016) Preliminary assessment of the population of Ixodes affinis (Ixodida: Ixodidae) in North and Central America. Systematic and Applied Acarology, 21, 1300–1308. https://doi.org/10.11158/saa.21.10.2
  28. Nadolny, R.M., Toliver, M., Gaff, H.D., Snodgrass, J.G. & Robbins, R.G. (2021) Focus stacking images of morphological character states for differentiating the adults of Ixodes affinis and Ixodes scapularis (Acari: Ixodidae) in areas of sympatry. Journal of Medical Entomology, 58, 1941–1947. https://doi.org/10.1093/jme/tjab058
  29. Neumann, L.G. (1899) Révision de la famille des ixodidés (3e mémoire). Mémoires de la Société Zoologique de France, 12, 107–294.
  30. Oliver, Jr J.H., Keirans, J.E., Lavender, D.R. & Hutcheson, J. (1987) Ixodes affinis Neumann (Acari: Ixodidae): New host and distribution records, description of immatures, seasonal activities in Georgia, and laboratory rearing. Journal of Parasitology, 73, 646–652. https://doi.org/10.2307/3282149
  31. Polsomboon, S., Hoel, D.F., Murphy, J.R., Linton, Y.M., Motoki, M., Robbins, R.G., Bautista, K., Bricen, I., Achee, N.L., Grieco, J.P., Ching, W.M. & Chao, C.C. (2017) Molecular detection and identification of Rickettsia species in ticks (Acari: Ixodidae) collected from Belize, Central America. Journal of Medical Entomology, 54, 1718–1726. https://doi.org/10.1093/jme/tjx141
  32. Rodríguez-Vivas, R.I., Apanaskevich, D.A., Ojeda-Chin, M.M., Trinidad-Martínez, I., Reyes-Novelo, E., Esteve-Gassent, M.D. & Pérez de León, A. (2016) Ticks collected from humans, domestic animals, and wildlife in Yucatán, Mexico. Veterinary Parasitology, 215, 106–113. https://doi.org/10.1016/j.vetpar.2015.11.010
  33. Ronquist, F., Huelsenbeck, J. & Teslenkoet, M. (2011) MrBayes Version 3.2 Manual: Tutorials and Model Summaries. Available from: http://mrbayes.sourceforge.net/manual.php (accessed 8 August 2023)
  34. Saracho-Bottero, M.N., Venzal, J.M., Tarragona, E.L., Thompson, C.S., Mangold, A.J., Beati, L., Guglielmone, A.A. & Nava, S. (2020) The Ixodes ricinus complex (Acari: Ixodidae) in the Southern Cone of America: Ixodes pararicinus, Ixodes aragaoi, and Ixodes sp. cf. I. affinis. Parasitology Research, 119, 43–54. https://doi.org/10.1007/s00436-019-06470-z
  35. Swofford, D.L. (2000) PAUP*: Phylogenetic Analysis Using Parsimony (and Other Methods) 4.0 Beta. Sinauer Associates, Sunderland, Massachusetts. [program]
  36. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739. https://doi.org/10.1093/molbev/msr121
  37. Venzal, J.M., Estrada-Peña, A., Barros-Battesti, D.M., Onofrio, V.C. & Beldoménico, P.M. (2005) Ixodes (Ixodes) pararicinus Keirans & Clifford, 1985 (Acari: Ixodidae): description of the immature stages, distribution, hosts and medical/veterinary importance. Systematic Parasitology, 60, 225–234. https://doi.org/10.1007/s11230-004-6351-9
  38. Wilson, D.E. & Reeder, D.M. (2005) Mammal species of the world: a taxonomic and geographic reference. 3rd Edition. Johns Hopkins University Press, Baltimore, Maryland, 2142 pp.
  39. Xu, G., Fang, Q.Q., Keirans, J.E. & Durden, L.A. (2003) Molecular phylogenetic analyses indicate that the Ixodes ricinus complex is a paraphyletic group. Journal of Parasitology, 89, 452–457. https://doi.org/10.1645/0022-3395(2003)089[0452:MPAITT]2.0.CO;2